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Figure 7


Fig. 7. LINT-25 acts upstream of LAP2{alpha}. (A) Primary skin fibroblasts from LAP2{alpha} knock-out mice were passaged in culture until senescence (S) and processed for immunofluorescence microscopy together with proliferating control cells (P) using antibodies against LINT-25, LAP2{alpha} or Ki67. (B) GFP-tagged LINT-25 (upper panel) or eGFP alone (lower panel) were transiently expressed in BJ1 cells and samples were processed for immunofluorescence microscopy 15 hours after transfection using antibodies against Ki67, LAP2{alpha} and Hoechst dye (blue). Confocal images are shown. Bars, 10 µm.





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