spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. LINT-25 localizes to the nucleus and associates with chromatin during mitosis. (A) HeLa cells were lysed in hypotonic buffer with iodoacetamide (IAA) (left panel), and total lysates (Pre), nuclear (N) and cytoplasmic (C) fractions were analyzed by immunoblotting using antibodies against LINT-25, lamin A/C and LAP2 (right panel). HeLa cells were lysed in culture dishes in buffer with the indicated concentrations of Triton X-100 and salt and total lysates (Pre), insoluble pellet fractions (P) and supernatants (S) were analyzed by immunoblotting. Note that the LINT-25 double band is caused by additional alkyl-groups resulting from the IAA treatment (see also supplement). Numbers indicate molecular masses of marker proteins in kDa; mLINT-25, monomeric LINT-25; dLINT-25, dimeric LINT-25; La, lamin. (B,C) HeLa cells were fixed with formaldehyde and double-stained for immunofluorescence microscopy using LINT-25 antiserum and antibodies against the indicated antigens. DNA was stained with Hoechst dye. Confocal images of interphase cells (B) or various stages of mitosis (C) are shown. Arrowheads indicate outer core regions. Bars, 10 µm.





Right arrow Return to article