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Figure 7


Fig. 7. Co-stimulation of actin assembly by IQGAP1 plus activated Cdc42, and inhibition of VCA-stimulated actin assembly by IQGAP1. (A) When used together, IQGAP1 and activated Cdc42 stimulated actin assembly rates additively, and virtually eliminated the lag period before peak assembly rates were reached using either IQGAP1 or activated Cdc42 alone. (B) In the presence of a GST-tagged, constitutively active, N-WASP VCA fragment, IQGAP1, but not IQGAP1{Delta}NT, caused dose-dependent inhibition of assembly.





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