spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. IQGAP1 is required for FGF2-stimulated migration of MDBK cells. (A) MDBK cells were transfected with IQGAP1-specific siRNA or scrambled RNA (scrRNA) using a Nucleofector. To allow direct visual comparison of IQGAP1 levels in the two samples by western blotting, a concentration series of each cell extract was analyzed at the indicated relative dilutions. Note that siRNA reduced the IQGAP1 level to ~20% of normal, but had no effect on cellular actin content. (B) Confluent monolayers were then serum-starved for 8 hours, wounded with a micropipette tip, and 2 hours later were stimulated with 25 ng/ml FGF2. Note that movement of IQGAP1-depleted cells into the wound, as seen after 7 hours of FGF2 exposure, was severely impaired, and that broad wounds persisted for more than 24 hours after wounding in both scrRNA-treated and IQGAP1-depleted cells that were not stimulated with FGF2.





Right arrow Return to article