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Fig. 5. Effect of OST subunit knockdown on the N-glycosylation of polytopic membrane proteins. (A) The extent of bovine opsin N-glycosylation was determined and quantified as before (Fig. 3A). (B) Rat neurotensin receptor N-glycosylation was determined as for A. The numbers below the lanes are the mean ± s.e.m. of three independent experiments. Levels of N-glycosylation that differ from the mock-treated control by a significance of at least 0.02 are indicated (*).