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Figure 1


Fig. 1. Consequences of siRNA-mediated knockdown of ribophorin I, STT3A and STT3B. (A) Lysates of cells 48 hours after transfection with ribophorin I, STT3A and STT3B siRNA duplexes (lanes 1-6), a non-functional siRisc-free control (siRF) (lane 7), or mock-transfected cells (lane 8) were probed with antibodies specific for ribophorin I (RibI), STT3A, STT3B or {alpha}-tubulin ({alpha}Tub). Mature forms of these proteins are indicated by arrows and the location of unglycosylated forms of ribophorin I and STT3A are shown by asterisks. For STT3B, the signal is relatively weak, and two non-specific products are also detected (filled circles). (B) Lysates of cells were prepared as in A except the RibIB siRNA duplex was omitted. Samples were probed using antibodies recognising OST48, ribophorin II (RibII), Dad1 or {alpha}-tubulin.





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