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Figure 3


Fig. 3. Colocalization of hCAF1 and PRMT1 with nuclear speckle marker proteins is sensitive to AMD treatment. Laser-scanning confocal microscopy of double-labeling experiments before and after treatment with AMD (5 µg/ml). Distribution in MCF-7 cells of endogenous hCAF1 (a) and PRMT1 (g) along with specific compartment proteins such as SC35 (b) and Sf3b (h) was analyzed by double-labeling experiments using laser-scanning confocal microscopy. Panels (c) and (i) show overlays of the two signals. Distribution of hCAF1 (d) and PRMT1 (j) along with SC35 (e) and Sf3b (k) on MCF-7 cells treated with AMD (5 µg/ml). The corresponding overlays are shown in panels (f) and (l).





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