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Figure 7


Fig. 7. IFT88/polaris interacts with Che-1 and inhibits its interaction with Rb protein. (A) HEK293T cells were transfected with control plasmid (pcDNA3.1A) or Myc-tagged full-length IFT88/polaris (pcDNA3.1A-Tg737). Lysates (500 µg) from transfected cells were immunoprecipitated with anti-Myc antibody or anti-Che-1 antibody and analyzed by western blot using specific anti-IFT88/polaris (740) or Che-1 antibodies. (B) Lysates (1 mg) from non transfected HEK293T cells were immunoprecipitated with anti-Che-1 antibody and analyzed by western blot using a specific anti-IFT88/polaris (740) antibody. (C) HEK293T cells were transfected with control plasmid or Myc-tagged full-length IFT88/polaris. Lysates (500 µg) from transfected cells were immunoprecipitated with anti-Che-1 antibody and analyzed by western blot using specific anti-Rb, anti-IFT88/polaris (740) or Che-1 antibodies. I.P., immunoprecipitation; I.B, immunoblot.





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