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Figure 6


Fig. 6. IFT88/polaris downregulation causes cells proliferation. (A) Two non-overlapping IFT88/polaris siRNAs (siPol1 and siPol2) were tested and were found to produce similar results. Data obtained with siPol1 are illustrated in this figure. HeLa cells were transfected with scramble or IFT88/polaris siRNA (siPol1). Cell lysates (48 hours post transfection) were subject to western blot (left panel) using either the anti-IFT88/polaris antibody 740 (top) or an anti-actin antibody (I-19, bottom). HeLa cells transfected with scramble or IFT88/polaris siRNA (siPol1) were also immunostained (right panel) with anti-IFT88/polaris antibody 740. (B) HeLa cells were transfected with scramble or siPol1, treated with BrdU during 4 hours before the indicated time and BrdU-positive cells were analyzed (n=300). (C) Growth curves of cells transiently transfected with scramble or siPol1. (D) FACS analysis, 48 hours post tranfection. The histogram indicates the percentage of cells at a given cell cycle phase. Data are from three independent experiments and presented as the mean ± s.d.





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