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Figure 2


Fig. 2. IFT88/polaris is localized to the centrosome throughout the cell cycle. Immunofluorescence images of endogenous IFT88/polaris in HeLa cells at different stages of cell cycle that stably express GFP-centrin1. Right panel, merged images of IFT88/polaris (red), centrin1 (green), and nuclei (blue) from cells in G1, G2, prophase, metaphase, anaphase and telophase. Insets, higher magnifications of centrioles stained for GFP-centrin1 (left) and IFT88/polaris (anti-IFT88/polaris 740, middle) in cells at G1 and G2. Bars, 5 µm.





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