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Figure 7


Fig. 7. Syt I but not Syt IX downregulation inhibits compensatory endocytosis. (A) Wild-type, Syt I-null, Syt IX-null and Syt I/IX-null cells expressing the human Tf receptor were incubated with Texas-Red-conjugated Tf in basal or depolarization medium for 20 minutes. Representative images for the uptake of Tf into the four indicated cells types resting in basal or depolarization medium are shown. Bar, 10 µm. (B) Z-stacks of confocal images were added up to give Tf integrated intensity per cell. The mean Tf integrated intensity ± s.d. is plotted for wild-type, Syt I/IX-null, Syt I-null and Syt IX-null cells (n=15 cells for each cell type) in basal or depolarization medium.





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