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Figure 3


Fig. 3. Loss of regulated exocytosis occurs at a late step downstream from Ca2+ entry. (A) Wild-type and clones stably expressing pSHAG-Syt I/IX (Syt I/IX-null) were transfected with a plasmid expressing hGH and permeable cell secretion assays were conducted (see Materials and Methods) in the absence (basal) or presence (Ca2+-stimulated) of 10 µM free Ca2+. Ca2+-dependent hGH secretion was determined as the amount of hGH secreted in 5 minutes at 30°C in Ca2+-stimulated minus basal conditions. Values represent the mean ± s.d. of triplicate determinations. (B) Ca2+ dose-response for exocytosis in wild-type and Syt I/IX-null cells. Secretion assays in permeable cells were conducted at the indicated Ca2+ concentrations in wild-type and Syt I/IX-null cells. hGH released was determined and plotted as the mean ± s.d. of triplicate determinations.





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