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Figure 1


Fig. 1. Syt I and Syt IX colocalize on DCVs in PC12 cells. (A) PC12 cells were fixed and stained with Syt I-specific (red channel) and Syt IX-specific (green channel) antibodies and coimmunolocalization was determined (yellow). 3D reconstruction of deconvolved confocal sections is shown in supplementary material Movie 1. Bar, 5 µm; square insets are 1 µmx1 µm. (B) Syt I and Syt IX colocalization was quantified in deconvolved confocal Z-sections of 12 randomly selected PC12 cells. Bar graphs give the total percent overlap (corrected for random overlap) for Syt I with Syt IX and Syt IX with Syt I as the mean ± s.d. (C) Representative deconvolved Z-section of two PC12 cells showing colocalization (yellow) of Syt I (red channel) and Syt IX (green channel). A 7-µm line was drawn through the edge of one PC12 cell for line-scan analysis. (D) Fluorescence intensity, as arbitrary fluorescence units of Syt I and Syt IX along the 7-µm line shown in C. *, individual DCVs. Fluorescence intensity of a 0.1-µm bead is shown for size comparison.





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