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Fig. 9. Effect of 4.1B on the motility of the sarcoma cells. (A) Non-metastatic K2 cells were injected with Cy3-labelled R-epb41l3 siRNA1 and recorded 48 hours later by low light level digital microscopy. Cell translocations were interactively tracked, and speeds of cell migration evaluated in Mathematica. Speed of cell migration was increased twofold in cells treated with siRNA targeting epb41l3 compared with those treated with control siRNA (ANOVA P<0.01; mean ± s.e.m. of normalised speed of cell migration was 1.0±0.1 and 2.2±0.2 for control siRNA and siRNA targeting Epb41l3, respectively; a total of 49 cells from ten time-lapse recordings was analysed). (B) GFP cDNA, or a mixture of GFP and 4.1B cDNA, were microinjected into metastatic T15 cells and after 5 hours cell movements recorded by low light level digital microscopy. Cells expressing both 4.1B and GFP migrated at half the speed of the cells injected with GFP alone (ANOVA P<0.01; mean ± s.e.m. of normalised speed of cell migration was 1.3±0.1 and 0.7±0.1 for GFP cDNA alone and for mixture of GFP and 4.1B cDNA, respectively; a total of 49 cells from nine time-lapse recordings was analysed).
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