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Figure 7


Fig. 7. Distribution of GFP-4.1B and focal adhesions in a T15 cell. The cell was microinjected with GFP-4.1B fusion construct, fixed after 5 hours and analysed under a laser scanning confocal microscope. (A-D) We simultaneously acquired an image of GFP-4.1B at the substrate level (A) and an interference reflection microscopy image (B) in which focal adhesions appear dark. C and D are enlarged images of boxed regions in A and B, respectively. They reveal enrichment of GFP-4.1B in many but not all focal adhesions. Arrows indicate examples of focal adhesions with an increased concentration of GFP-4.1B. Bar, 20 µm.





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