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Fig. 3. Differences in F-actin organisation among the sarcoma cell populations. (A) These single confocal images of Rhodamine-phalloidin-stained cells reveal differences among the sarcoma cell populations. Lower magnification images (right panels) demonstrate that these phenotypes are representative across a larger number of cells. F-actin in K2 (non-metastatic cells) is arranged into stress fibres, which can be quite thick and can traverse the cell. T15, A297 and A311 cells typically have F-actin concentrated into ruffles at the leading edge of the cell; elsewhere it is arranged cortically and in a disordered manner. These cells are often polarised. Bars, 20 µm. (B) The percentage of actin stress fibre-containing cells in each population. Quantification of randomly acquired fields of cells revealed that 89±8% of K2 cells contain at least one stress fibre, compared with only 7±2%, 3±8% and 7±8% of T15, A297 and A311 cells, respectively. The numbers of cells scored for K2, T15, A297 and A311 were 34, 84, 58 and 69, respectively. The individual differences in stress fibre distribution between the T15, A297 and A311 compared with K2 cells are all significant (***P<0.001).
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