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Figure 2


Fig. 2. Motile responses of the sarcoma cells to PDGF-IGF. (A) Distribution of directions of cell translocation in a gradient of PDGF-IGF in the Dunn chemotaxis chamber is represented by circular histograms. The circular histograms are aligned such that the concentration of PDGF-IGF in the gradient increases vertically towards the top. Translocation of a cell from its starting point was evaluated and its direction was taken on migrating 70 µm. Cells not migrating 70 µm were not included in this analysis. Significant unimodal clustering of directions according to the Rayleigh test is indicated by an arrow identifying the mean direction, and a shaded wedge representing its 95% confidence interval. K2 cells are weakly chemotactic to PDGF-IGF, whereas T15, A297 and A311 cells have strong chemotactic responses (P<0.005). Data from left to right are from K2, T15, A297 and A311 cells and represent 5, 4, 5 and 5 independent experiments, respectively, each recorded over a period of 16 hours. The total numbers (fractions) of K2, T15, A297 and A311 cells included in the analysis are 61 (36%), 81 (70%), 86 (49%) and 47 (42%), respectively. (B) Histograms to illustrate the distribution of speed of cell migration within the four cell populations. The non-metastatic K2 cells moved at 11.1±0.5 µm/hour, whereas the metastatic T15 and A297 cells moved significantly faster,at 17.1±0.7 µm/hour (ANOVA P<0.05) and 19.1±0.9 µm/hour (ANOVA P<0.01), respectively. There was no significant difference between the speed of cell migration in K2 and A311 cells, which migrated at 14.1±0.9 µm/hour. However, the proportion of cells moving faster than 20 µm/hour was significantly increased ({chi}2 P<0.01) in A311 cells (20%) compared with K2 cells (8%). The speed data were also derived from the experiments with the Dunn chemotaxis chamber without excluding any cells. The total numbers of K2, T15, A297 and A311 cells were 171, 116, 176 and 113, respectively, and numbers of 5-minute displacements were 21340, 13390, 20179 and 15436, respectively.





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