JCS00273 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 (Adobe PDF) -

  Fig. S1. Algorithms used for processing the microarray data was developed in Mathematica (Wolfram Research, Inc.). Briefly, the algorithms performed the following functions: loading data (A); exclusion of genes whose expression was undetectable on each replicate, normalization calculation of mean expression value from biological replicates; calculation of relative expression between samples; calculation of statistical significance (B); production of ranked table of candidate genes (C); (D) presents definition of functions used.

• Supplemental Figure 2 (Adobe PDF) -

  Fig. S2. A third splice variant of Epb41l3 was identified. The sequence of the new variant, GenBank Accession Number DQ462202, was aligned with the sequence of the major isoform, NM_053927, using the Clustal W multiple sequence alignment program (Chenna et al., 2003). (A) The multiple sequence alignment of the non-homologous regions is shown; base numbers correspond to NM_053927. The sequence of DQ462202 diverges from the sequence of NM_053927 at bases 1569-2115, 2169-2204 and 2398-2521. (B) The chromatogram of the DQ462202 sequence covering these regions; the sequence numbers of the chromatogram correspond to DQ462202.