JCS03354 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. As for females, immunisation against the N-terminal domain of the NMDA receptor NR1 subunit prevents the neurotoxic effect of endogenous tPA in males. Focal permanent ischemia was induced by MCA occlusion in mice. Lesion volumes 24 hours post ischemia were analysed by Student’s t-test (n=5-6 per group, mean ± s.e.m.; **P<0.01).

• Supplemental Figure 2 -

  Fig. S2. Immunisation-induced impairment of spatial memory is transient. Spatial memory was assessed, 13-14 days (A) or 36-37 days (B) after the last injection, by the ability to discriminate the novel arm (calculated based on the following formula: recognition of the novel arm=absolute time spent in the novel arm − average time spent in arms 1 and 2). The discrimination ratio was analyzed by a one-way ANOVA (n=9-11 in each group), *P<0.05.

• Supplemental Figure 3 -

  Fig. S3. Absence of gender influence on spatial memory in wild-type mice. Spatial memory was assessed in male and female Swiss mice (n=15 in each group) by the ability to discriminate the novel arm (calculated based on the following formula: recognition of the novel arm=absolute time spent in the novel arm − average time spent in arms 1 and 2). No difference was observed in the discrimination ratio of both groups (mean±s.e.m.; P=0.94).

• Supplemental Figure 4 -

  Fig. S4. Absence of gender-specific influence on tPA-dependent deficit in spatial memory. Spatial memory, assessed by the ability to discriminate the novel arm (calculated based on the following formula: recognition of the novel arm=absolute time spent in the novel arm − average time spent in arms 1 and 2) was not different in male (n=14) and female (n=20) tPA-deficient mice (mean ± s.e.m.; P=0.42).