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Figure 8


Fig. 8. The expressions of RNAiR-IQGAP2 and - IQGAP3 rescue axon outgrowth. (A) The neurons were cotransfected with the indicated siRNA and RNAiR-IQGAP3, followed by immunostaining with anti-Myc (green) and Tau-1 (red) antibodies at DIV3. Arrowheads indicate the cotransfected cells that were identified by the anti-Myc antibody. RNAiR-IQGAP3 rescued the impaired axon outgrowth created by siIQGAP2 or siIQGAP3. The results represent five independent experiments. Scale bar equals 20 µm. (B) Quantification of the length of the longest primary axon. The axon length was measured in the cells cotransfected with the indicated combinations. Data represent the means ± s.d. of five independent experiments. n>150. Asterisks indicate a significant difference from the value of the control cells at P<0.01.





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