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Figure 2


Fig. 2. IQGAP3 is an effector for Rac1 and Cdc42. (A) IQGAP3-V5Hisx6 was mixed with the affinity beads coated with the indicated GST-fused small GTPases. Bound IQGAP3-V5Hisx6 was co-eluted with GST-fused proteins by the addition of glutathione. The eluates were subjected to SDS-PAGE, followed by immunoblot analysis with anti-His antibody (top) and silver staining (bottom). The results shown are representative of three independent experiments. (B) COS7 cells expressing EGFP-IQGAP3 were lysed, and the supernatants were incubated with the beads coated with the indicated GST-fused small GTPases. The eluates were subjected to SDS-PAGE, followed by immunoblot analysis with anti-GFP antibody (top) and silver staining (bottom). The results shown are representative of three independent experiments.





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