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Figure 4


Fig. 4. Both endogenous and exogenous Rac1 and Rac3 localize to different intracellular compartments. (A) N-terminally eGFP-coupled Rac1 or -Rac3 was expressed transiently in N1E-115 cells. Cells were subsequently serum-starved for 8 hours, fixed and stained with phalloidin to visualize filamentous actin. Bar, 25 µm. (B) N1E-115 cells cultured under normal conditions were fixed and stained with anti-Rac1 or anti-Rac3 antibodies. (C) N-terminal eGFP-coupled Rac3*CAAX1 and Rac3*Cterm1 mutants were expressed in N1E-115 cells to monitor their intracellular localization.





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