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Figure 8


Fig. 8. Live-cell recordings show SvkA in the midzone. (A) Wild-type cells expressing GFP-SvkA-FL accumulate the kinase only after the appearance of the cleavage furrow. The arrows indicate the localization of SvkA in the midzone during cell separation. (B) A svkA-knockout cell (confocal section of live cell, agar overlay) containing four nuclei (stars) and expressing the CT-terminal domain of SvkA. GFP-SvkA-CT determines localization to the midzone but is not sufficient to prevent incomplete cell division (see renewed fusion of partially separated cells in frame 11'). (C) SvkA-knockout cells expressing the dead kinase show that GFP-SvkA-K134A-FL localizes to the late cleavage furrow (arrows). (The stars indicate the approximate localization of the nuclei outside the focus plane. Bars, 5 µm.)





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