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Figure 7


Fig. 7. Enrichment of SvkA around the centrosome, the spindle and the midzone. (A) SvkA-knockout cells expressing GFP-SvkA-FL were fixed and stained with antibodies against alpha-tubulin. Three confocal sections are shown. GFP-SvkA-FL is enriched diffusely around the centrosome of interphase cells (open arrow) and around the spindle in mitotic cells (closed arrows). (B) Confocal section of fixed wild-type cells expressing the C-terminal domain construct GFP-SvkA-CT. The arrow indicates the localization of GFP-SvkA-CT around the centrosome. (C) A confocal section of fixed wild-type cells expressing GFP-SvkA-FL shows colocalization with the DNA (upper panel) and, at later stages, around the spindle (lower panel). Bars, 5 µm.





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