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Figure 7


Fig. 7. Recruitment of Cep68 but not Cep215 to rootletin fibres. Tagged constructs of rootletin, Cep68 and Cep215 were overexpressed in U2OS cells and filament formation as well as the recruitment of endogenous proteins to induced filaments was examined by immunofluorescence microscopy. Bars, 10 µm. (A) GFP-rootletin (left) was coexpressed with Myc-Cep68 or Myc-Cep215 (centre); merged images are shown on the right (GFP-rootletin in green; Myc in red). Note that Myc-Cep68 but not Myc-Cep215 colocalises with rootletin fibres. (B) Overexpressed Myc-Cep68 (centre) colocalises with endogenous rootletin at the centrosome (centre) but does not form polymers; merged image on the right shows Myc-Cep68 in green and rootletin in red. (C) Endogenous Cep68 (centre) colocalises with Myc-rootletin (left) overexpressed to low (top) and high (middle) levels, but endogenous Cep215 is not recruited to Myc-rootletin filaments (bottom); merged images on the right show Cep68 and Cep215 in green and Myc-rootletin in red.





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