spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Distinct associations of Cep68 and Cep215 with centrosomes. Antibodies directed against Cep68 (A) or Cep215 (B) and corresponding pre-immune sera were used for immunofluorescence on U2OS cells. The antibodies (green) recognise the centrosome as indicated by colocalisation with {gamma}-tubulin (red). Insets show enlarged areas to better visualise the centrosomes; note filamentous staining produced by anti-Cep68 antibody and compact staining produced by anti-Cep215 antibody. Pre-immune sera did not show any specific staining (top panels) and 48 hour siRNA treatment using duplex 262 targeting Cep68 or duplex 283 targeting Cep215 abolished Cep68 (A, bottom) and Cep215 signals (B, bottom), respectively. Note the centrosome splitting induced by siRNA-mediated depletion of either Cep68 or Cep215. Bars, 10 µm.





Right arrow Return to article