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Figure 8


Fig. 8. Lsm7p and Lsm8p delocalize after hyperosmotic shock, but not after glucose deprivation. MRY74 (LSM7-13myc), MPS3 (LSM8-13myc), MPS15 (xpo1-1, LSM7-13myc) and MPS16 (xpo1-1 LSM8-13myc) cells were grown at 30°C in YPDA to log phase and shifted to medium containing galactose (Gal), no glucose (YP) or 1 M KCl. Cells were fixed after 15 minutes of incubation at room temperature and examined by immunofluorescence. (A) Localization of Lsm7-13myc (red in merge); P-bodies indicated by arrows. (B) Localization of Lsm8-13myc (red in merge). (C) To measure the decay rate of Lsm7p, extract from AEMY35 (PGAL1-HA-LSM7) cells harvested at 1, 2, 4, 6, 8 and 10 hours after shifting from galactose to glucose medium was tested by western blot analysis with anti-HA antibodies. (D) BMA38a with pMPS2 (GFP-Lhp1) was grown in SD-Ura-Met to log phase and shifted to 1 M KCl. Cells were fixed before and after 15 minutes in 1 M KCl. GFP-Lhp1 is shown in green; DAPI in red.





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