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Figure 7


Fig. 7. Overexpression of LSM1 in an lsm8-1 background results in a severe slow-growth phenotype. (A) Growth curve of wild-type (WT; MPS2) and lsm8-1 (MPS17) strains with or without over-production of Lsm1p (1oe). Wild-type yeast with Lsm7p myc-tagged (MPS2) or lsm8-1 yeast with Lsm7p myc-tagged (MPS17) were transformed with either pBM125 (empty vector) or pAEM80 (PGAL1-HA-LSM1). All strains were grown in galactose-based media. (B) Northern blot showing the levels of U1 and U6 snRNAs in wild-type or lsm8-1 cells combined with over-production of Lsm1p. (C) Localization of Lsm7-13myc in wild-type and lsm8-1 strains, with or without over-production of Lsm1p. All strains were grown in galactose and the localization of Lsm7p was detected with anti-Myc antibodies. Representative cells are shown. Arrows indicate `holes' in the immunofluorescence at the positions of nuclei. Bars, 10 µm.





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