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Figure 6


Fig. 6. Over-production of Lsm1p or Lsm8p has opposing effects on Lsm7p localization. (A) HA-tagged Lsm1p or Lsm8p was over-produced from plasmid pAEM80 or pAEM76, respectively, in strain MPS2 (Lsm7-13myc) grown in SDGal-Ura. Lsm7-13myc localization (red in merge) in wild type (WT; MPS2 carrying pBM125 empty vector), or with LSM1 or LSM8 overexpression (o/e LSM1 and o/e LSM8, respectively). Over-production of Lsm1p caused reduction of nuclear Lsm7p (large arrow) and its accumulation in foci (small arrows). Bar, 10 µm. (B) Lsm8p over-production does not affect Lsm1p levels. MPS1 was grown with pBM125 or pBM125-HA-LSM8 in SD-Ura (Glu) or SDGal-Ura (Gal). Total protein was separated by SDS-PAGE and the western blot was probed for Lsm1-13myc, HA-Lsm8 and {alpha}-Tubulin.





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