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Figure 2


Fig. 2. Localization of the Lsm proteins in the nup49-313 nuclear-import mutant and the xpo1-1 export mutant. Cultures were grown at 23°C to log phase and then at 37°C for a further 4.5 hours. In each case, a representative cell is shown. (A) Localization of Lsm7-13myc in MPS2 (wild type, WT) and MPS12 (nup49-313) at 23°C and 37°C. (B) Localization of Lsm8-13myc in MPS3 (WT) and MPS13 (nup49-313) at 23°C and 37°C. (C) Fluorescent in situ hybridization showed that localization of U1 snRNA in nup49-313 yeast at restrictive temperature was the same as in wild-type cells. (D) Localizations of Lsm1-13myc, Lsm7-13myc and Lsm8-13myc at 37°C in the xpo1-1 mutant strains MPS13, MPS14 and MPS15, respectively, were the same as for wild-type cells. Arrows indicate `holes' in the immunofluorescence at the positions of nuclei. Bars, 10 µm.





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