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Figure 1


Fig. 1. Localization of Lsm1p, Lsm7p and Lsm8p. (A) Western blot showing 13myc-tagged Lsm1p, Lsm7p and Lsm8p from yeast strains MPS1, MPS2 and MPS3, respectively. (B) Localization of Lsm1-13myc, Lsm7-13myc and Lsm8-13myc in strains MPS1, MPS2 and MPS3, respectively. Fixed cells were stained with anti-Myc antibodies followed by Cy3-conjugated secondary antibody (red in merge). Nuclei were stained with DAPI (green in merge). For clarity, single channel images of DAPI and of immunofluorescence are shown in greyscale. Arrows indicate `holes' in the immunofluorescence at the positions of nuclei. Bars, 10 µm.





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