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Figure 5


Fig. 5. Direct interaction of Tyr and Tyrp1. Tyr was immunoprecipitated from melan-a melanocytes pretreated with or without DSP, and was separated in the first dimension by SDS-PAGE under nonreducing conditions. Tyr and crosslinked proteins were cleaved and resolved in the second dimension by SDS-PAGE under reducing conditions. Blots of DSP-treated samples detected by anti-TyrGP (green) and Tyrp1 GP (red) were overlaid and are shown in the bottom panel. Arrowheads indicate Tyr or Tyrp1 detectable only when cells had been pretreated with DSP. Tyr spots marked with arrows were detected regardless of DSP treatment and probably represent Tyr that comigrated with the anti-Tyr IgG heavy chain on the first gel. There are also some non-specific spots visible at ~58 kDa. Representative results of two experiments are shown.





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