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Figure 3


Fig. 3. (A-L) Subcellular localization of selected monomeric FP fusions (listed in Table 1) with targeting proteins imaged in widefield fluorescence. Images are pseudocolored to match the FP emission profile. The FP fusion terminus and number of linker amino acids is indicated after the name of the targeted organelle or fusion protein. (A) EBFP2-mito-N-7 (human cytochrome C oxidase subunit VIII; mitochondria); (B)mCerulean-paxillin-N-22 (chicken; focal adhesions); (C) mTFP1-actin-C-7 (human β-actin; filamentous actin); (D)mEmerald-keratin-N-17 (human cytokeratin 18; intermediate filaments); (E) superfolder GFP-lamin B1-C-10 (human lamin B1; nuclear envelope); (F)mVenus-Cx43-N-7 (rat {alpha}-1 connexin-43; gap junctions); (G) YPet-EB3-N-7 (human microtubule-associated protein; RP/EB family); (H) mKO-Golgi-N-7 (N-terminal 81 amino acids of human β-1,4-glactosyltransferase; Golgi complex); (I) tdTomato-zyxin-N-7 (human zyxin; focal adhesions); (J) TagRFP-tubulin-C-6 (human {alpha}-tubulin; microtubules); (K)mCherry-vimentin-N-7 (human vimentin; intermediate filaments); (L)mPlum-{alpha}-actinin-N-19 (human non-muscle; cytoskeleton). (M-Q) Fusion of mEGFP with human histone H2B (mEGFP-H2B-N-6). (M) interphase; (N)prophase; (O) prometaphase; (P)metaphase; (Q) anaphase.





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