JCS012468 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 - Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. (a) Serial sections of DPM540 cells showing simultaneous localisation of microtubules (green) and Myo52 (red) in live cells. Myo52 is not seen to associate with microtubules throughout the cell. (b) Serial sections of DPM602 cells showing simultaneous localisation of actin filaments (green) and Myo52 (red) in live cells. Motile Myo52 foci are always seen associated with actin filaments.

• Movie S1 -

  Movie 1. 4D Timelapse movie of myo52-cgfp cells showing Myo52 foci moving throughout the cytoplasm.

• Movie S2 -

  Movie 2. 4D Timelapse movie simultaneously showing Myo52 and microtubule dynamics in myo52-cgfp cells and gfp-atb2 cells (arrows) in the presence of DMSO.

• Movie S3 -

  Movie 3. 4D Timelapse movie simultaneously showing Myo52 and microtubule dynamics in myo52-cgfp cells and gfp-atb2 cells (arrows) in the presence of 25 μg/ml carbendazim.

• Movie S4 -

  Movie 4. 4D Timelapse movie simultaneously showing Myo52 and actin-patch dynamics in myo52-cgfp cells and crn1-gfp cells (arrows) in the presence of DMSO.

• Movie S5 -

  Movie 5. 4D Timelapse movie simultaneously showing Myo52 and actin-patch dynamics in myo52-cgfp cells and crn1-gfp cells (arrows) in the presence of 1μM latrunculin A.

• Movie S6 -

  Movie 6. 4D Timelapse movie simultaneously showing Myo52 and actin-patch dynamics in myo52-cgfp cells and crn1-gfp cells (arrows) in the presence of 20 μM latrunculin A.

• Movie S7 -

  Movie 7. 4D Timelapse movie of myo52-cgfp for3Δ cells showing that Myo52 fails to recruit to motile foci associated to the interphase actin filament array in the absence of the formin For3.

• Movie S8 -

  Movie 8. 4D Timelapse movie of DPM854 cells showing GFP-Myo52ΔIQ Myo52 foci moving throughout the cytoplasm.