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Figure 2


Fig. 2. Genetic interactions of erg4{Delta}, cbr1{Delta} and ncp1{Delta}. (A) Deletion of CLA4 in erg4{Delta} cells or ncp1{Delta} is lethal. Serial dilutions (1:10) of the indicated strains were spotted on YPD and 5-FOA plates and grown for 2 days at 30°C. Notice that cla4{Delta} erg4{Delta} and cla4{Delta} ncp1{Delta} carried the plasmid pRS316-CLA4, whereas cla4{Delta} ste20{Delta} carried the plasmid pRS316-STE20. 5-FOA selects against these URA3-based pRS316 derivatives. (B) STE20 deletion reduces the growth of ncp1{Delta} cells. Serial dilutions of the indicated strains were spotted on YPD and 5-FOA plates as described in A. (C) Genetic interactions with och1{Delta}. Cells were spotted as in A.





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