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Figure 5


Fig. 5. Stimulation of lentoid formation and levels of lens differentiation markers filensin and CP49 by wild-type and loss-of-gap-junction mutants of Cx45.6. (A) Lens primary cultures were infected with recombinant retroviruses RCAS(A), RCAS(A)-Cx45.6, RCAS(A)-Cx45.6(D47A) or RCAS(A)-Cx45.6(P88S) for 8 days and the total numbers of lentoids were quantified. (B) Lysates from cells infected with retroviruses RCAS(A) vehicle (lane 1), RCAS(A)-Cx45.6-WT (lane 2), RCAS(A)-Cx45.6(D47A) (lane 3), and RCAS(A)-Cx45.6(P88S) (lane 4) were loaded on SDS-PAGE gels and immunoblotted with antibodies against filensin, CP49 or beta-actin. (C) The CP49 protein bands from three separate western blot analyses were quantified by densitometry. *P<0.05, in comparison with the non-Cx45.6-overexpressing control.





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