spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 9


Fig. 9. Wsh3/Tea4 recruits Dis2.NEGFP to cell tips. (A) dis2.NEGFP wsh3+ (IH2089) cells were dipped in red lectin and mounted alongside dis2.NEGFP wsh3.{Delta} (IH5722) cells. Continuous imaging of GFP fluorescence in three consecutive z slices (compressed into a single maximum projection) showed that the Dis2.NEGFP cap structure seen at the end of dis2.NEGFP wsh3+ cells was absent when wsh3+ was deleted (three left panels, see Movie 5 in supplementary material). Depolymerisation of microtubules by the addition of 25 µg/ml CBZ abolished Dis2.NEGFP cap signals in both the wsh3+ and the wsh3.{Delta} backgrounds (right panel). (B) Consecutive green and red images of dis2.NEGFP wsh3.C2tdTom (IH5726) cells were continuously taken in three consecutive z stacks to reveal the dynamics of the association of Dis2.NEGFP with Wsh3.C2tdTom at cell tips. See Movie 6 in supplementary material. Bars, 5 µm.





Right arrow Return to article