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Figure 6


Fig. 6. Dis2.NEGFP associates with both growing and non-growing tips. dis2.NEGFP (IH2908; A,B), dis2.NEGFP cdc10.129 (IH2345; C) and dis2.NEGFP cdc25.22 (IH2356; D) cells were processed as for Fig. 1. (A) Prior to mounting, dis2.NEGFP cells were re-suspended in TRITC-lectin so that the entire cell surface became fluorescent. Washing this lectin out by repeated changes of medium and subsequent growth resulted in dark non-stained tips, identifying those tips that were actively growing and those that were not (stained tips). (B) Dis2.NEGFP foci associated with the cell tips during interphase, mitosis and immediately after cytokinesis. Note the lower Dis2.NEGFP signal intensity at the tips of mitotic cells. (C,D) Dis2.NEGFP associated with both cell tips in cells that are arrested before NETO at START (C) and after NETO at the G2-M boundary (D). Bars, 5 µm.





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