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Figure 4


Fig. 4. Dis2.NEGFP recruitment to cell tips requires microtubules. dis2.NEGFP (IH2908; A) and a mix of dis2.NEGFP (IH2908) and nmt81.GFP.atb2 (IH1741) cells (B-D) were processed as for Fig. 1 with the exception that DMSO containing CBZ, to a final concentration of 25 µg/ml, was included in the culture medium for mounting in C and D and an equivalent amount of DMSO was added to the culture in B. Whereas Dis2.NEGFP distribution was not affected by the addition of DMSO (B), disruption of the microtubule cytoskeleton (verified by the lack of any microtubule signals in the control nmt81.GFP.atb2 cells, asterisks) abolished the association of Dis2.NEGFP with cell tips (compare inset in B to that in D). See also Movies 3 and 4 in supplementary material. The Dis2.NEGFP dots that are seen away from the cell tip were not affected by microtubule depolymerisation. Bar, 5 µm.





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