spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Sds21.NEGFP accumulates in nuclei, dots within the nucleus and at the cell periphery. Cells of the indicated strains were grown as for Fig. 1 with (A-C) or without (D,E) 10 µg/ml Hoechst 33342. In D and E the strains indicated in the central panel were transiently exposed to TRITC-lectin before being mixed and mounted with the deletion strain. (A,B) Sds21.NEGFP accumulates in nucleoli of dis2+ cells (IH2352) and is recruited to all sites normally occupied by Dis2.NEGFP when the dis2+ gene has been deleted (IH2635). (C) The distribution of Dis2.NEGFP is not affected by deletion of sds21+(IH2090). (D) Imaging sds21.NEGFP cells that have been dipped in red lectin (central micrograph) alongside sds21.NEGFP dis2.{Delta} cells shows an increase in the intensity of the Sds21.NEGFP signal upon deletion of dis2+. (E) Imaging dis2.NEGFP cells that have been dipped in red lectin (central micrograph) alongside dis2.NEGFP sds21.{Delta} cells shows no increase in the intensity of the Dis2.NEGFP signal upon deletion of sds21+. (F) Western blotting with antibodies to GFP establishes that the increase in Sds21.NEGFP signal intensity in dis2.{Delta} arises from an increase in protein levels. A-E. Bar, 5 µm.





Right arrow Return to article