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Figure 1


Fig. 1. Dis2.NEGFP accumulates in nuclei, dots within the nucleus and at the cell periphery. dis2.NEGFP (IH2908) cells were grown to mid-log phase in EMM2-FS and mounted in EMM2-FS with (B) or without (A,C) 10 µg/ml Hoechst 33342. In A the inset shows Dis2.NEGFP on both sides of a primary septum. In B and C the insets show enlargements of the indicated nuclei. In C the green arrows indicate the accumulation of Dis2.NEGFP in the cytokinetic actin ring during anaphase. See also Movies 1 and 2 in supplementary material. Bars, 5 µm.





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