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Fig. 5. Centrosomal abnormalities are present from the first mitotic cycle. (A) Mitosis in cycle 1 in wild-type Oregon embryos. DNA (DAPI, blue), microtubules (-tubulin, green), centrosomes (Cnn, red). Centrosome separation occurs at telophase in wild-type embryos. (B) Cycle 1 mitosis in mcph1^d2/d2 embryos. Nuclear compaction is less advanced at pronuclear fusion and prophase in mutant embryos (larger nuclear diameter than in wild type). Duplication of Centrosomin signal is also apparent from prophase, much earlier than in wild-type embryos. By metaphase, centrosomes are tandemly duplicated along the axis of the spindle, rather than side by side. Centrosome detachment also occurs, and increased -tubulin is seen at the centrosome, compared to that in wild-type embryos. The mitotic spindle is irregular and less substantial. Bar, 10 µm. (C) Centrosomal separation occurs earlier in mcph1^d2/d2 embryos than wild-type Oregon cycle 1 embryos. n=66 mcph1, n=22 Oregon. (D) Proportion of cycle 1 embryos by mitotic stage, from 0-30 minute AED embryos collections of wild-type and mcph1^d2/d2 females. There is an excess of mcph1^d2/d2 embryos at prophase and metaphase in cycle 1 (P<0.001). Error bars, standard error. mcph1, total embryos scored n=601, unfertilised n=125, meiosis n=66, cycle 1 n=179, cycle 2 and later n=231 (38.44%). Oregon, total embryos scored n=656, unfertilised n=81, meiosis n=55, cycle 1 n=179, cycle 2 and later n=418 (63.72%).

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