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Figure 3


Fig. 3. ADAMTS12 negatively regulates the HGF signalling pathway. (A) Levels of different components of the HGF pathway (E-cadherin, vimentin, ERK, P-ERK, AKT, P-AKT and P-Met) were evaluated by western blot in the selected cMDCK, TS12-4 and TS12-22 clones, in both non-stimulated (– HGF) and stimulated (+ HGF) conditions. Ectodomain shedding of Met was also analyzed in the conditioned medium from these clones (Met). (B) Immunostaining of E-cadherin and vimentin in the selected clones without (– HGF) and with (+ HGF) stimulation by hepatocyte growth factor. Bar, 10 µm.





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