|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 3. Kinetics of transferrin binding to its receptor and internalization. (A) Imaging of a T cell migrating on ICAM-1, 2 minutes after Tfn-Tx addition, at 60 second intervals. DIC images of the first and last frame are shown. Arrowheads point to the trailing uropod. (B,C) Kinetics of Tfn-A488 distribution 1 and 4 minutes after addition, respectively, and co-localization analysis with membrane-TfnR in non-permeabilized T cells. In this analysis, Tfn/TfnR co-localizing pixels correspond to surface-associated Tfn. Scatter plots display the intensity distribution and degree of co-localization corresponding to the entire cell, which is shown next to it. The enclosed areas (yellow rectangles) determine the co-localizing pixels, overlaid in white on the merged images. Magnification of Tfn/TfnR at the uropod membrane is shown at 4 minutes (the enlarged area is depicted in blue). Images at the 4th (20/20) and the 1st minute (16/20) are totally and widely representative, respectively. In the last case, 20% of the cells already showed a polarized distribution of membrane-TfnR/Tfn after 1 minute. The evolution of Tfn/TfnR distribution can also be observed in intensity profiles along membrane vectors from the uropod to the front pole (yellow lines). Bars, 5 µm.
|
