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Figure 8


Fig. 8. Mitch is a component of the Ndc80 complex. (A) Localization of TAP-tagged Mitch protein expressed in Drosophila Kc tissue culture cells. Mitch fused with protein-A–CBP either at the N-terminus (NTAP) or at the C-terminus (CTAP) localizes to the kinetochores of the chromosomes (blue). Bar, 10 µm. (B) Mitch co-purifies with Hec1, Nuf2, Mis-12 and CG1558 (arrows). SDS-PAGE gels were stained with Coomassie Blue and protein bands were identified by mass spectrometry. Other bands on the gel represent proteins that are either highly abundant (tubulin, actin), also present in the control (Kc cells without the tagged construct), or breakdown products of the proteins present in the complex.





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