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Figure 9


Fig. 9. Immunofluorescent localization of P-Smad2 in the coronary artery from wild-type and ApoE-null mice fed a high cholesterol diet. (A,B) Representative photographs of the coronary artery from wild-type (A) mice exhibited a plaque-free section; that from ApoE-null mice fed a high cholesterol diet (B) showed an advanced plaque. (C,D) Immunofluorescent confocal microscopic analysis of the tissue cross sections revealed that P-Smad2 is present in wild-type mice (C) whereas no P-Smad2 was detected in the endothelium of the coronary artery from ApoE-null mice fed a high cholesterol diet (D). *The location of the artery lumen. The magnification is 200x (A and B); bar, 20 µm (C,D). The arrows in C indicate the localization of P-Smad2 in the artery endothelium.





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