spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online September 19, 2007

Journal of Cell Science 120, 1902e (2007)
© The Company of Biologists Limited
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JCS
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Search for Related Content

In this issue

mDia2 – the lamella's formin'


Figure 1

Cell migration relies on the dynamics of two overlapping F-actin networks – one at the edge of the cell (the lamellipodium) and one a few micrometers below the surface (the lamella). Actin-associated proteins regulate both these networks. On p. 3475, Clare Waterman-Storer and colleagues show that one such protein, mammalian Diaphanous-related formin (mDia2), a key nucleator of actin filaments, is crucial for regulating several aspects of F-actin dynamics in the epithelial cell lamella. Studying live cells, they show that mDia2 associates with the actin network in the lamella – but not the lamellipodium. They have blocked mDia2-mediated actin polymerisation by using antibodies or dominant negative constructs and followed the dynamics of F-actin by quantitative fluorescent speckle microscopy (which tracks randomly incorporated fluorescently tagged actin within a polymer). They find that mDia2 affects the stability and organisation of the lamella and is needed to maintain a stable pool of polymerization-competent F-actin filaments at focal adhesions (the structures that link the actin network to the substrate). Interestingly, mDia2 affects the kinetics of F-actin in both the lamella and lamellipodium; mDia2 at focal adhesions might thus somehow regulate the differential polymerisation of these two networks.


Related articles in JCS:

mDia2 regulates actin and focal adhesion dynamics and organization in the lamella for efficient epithelial cell migration
Stephanie L. Gupton, Kathryn Eisenmann, Arthur S. Alberts, and Clare M. Waterman-Storer
JCS 2007 120: 3475-3487. [Abstract] [Full Text]  




This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JCS
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Search for Related Content