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Figure 1


Fig. 1. Knockdown of mDia1 suppresses SRF activity, MyoD expression and cell cycle progression. (A) Quantitative real-time RT-PCR analysis of mDia1 mRNA in cells transfected with control GFP shRNA (sh-GFP) and mDia1 shRNA (sh-mDia). Values represent normalized fold differences between mDia1 and GAPDH mRNA in each sample (n=3) ± s.d. (B,C) MyoD expression in myoblasts co-transfected with empty vector or mDia1 shRNA and a GFP reporter, 24 hours after transfection (mean ± s.d., n=4, P<0.0013). (D) Knockdown of mDia1 reduces SRF activity. Normalized SRF activity in C2C12 myoblasts co-transfected with mDia1 shRNA or empty vector, the SRF reporter 3D.Aluc and beta-gal (mean ± s.d., n=4, P<0.0001). (E) FACS analysis of mDia1-knockdown cells shows an increased G1 population compared with control cells.





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