spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Depolarization induces nuclear accumulation of PYK2 in PC12 cells. (A) PC12 cells were treated with high [K+]o for 3 minutes or 11 ng/ml leptomycin B for 3 hours. Endogenous PYK2 was detected with an anti-PYK2 antibody and nuclei were stained with DAPI. Bars, 10 µm. (B) Percentage of cells with PYK2 immunoreactivity in the nucleus that was less than (n<c), equal to (n=c) or greater than (n>c) that in the cytoplasm. Values are means ± s.e.m., {chi}2=44.9, d.f.=4, P<0.0001, control vs K+ P<0.0001, control vs lepto B P<0.1, K+ vs lepto B P<0.0001. (C) Cells were immunostained with a pY402-PYK2 phospho-specific antibody and analyzed with confocal microscopy. Bars, 10 µm. (D) Quantification as in B was done for pTyr402-PYK2 immunoreactivity. Values are means ± s.e.m. {chi}2=623.2, d.f.=4, P<0.0001.





Right arrow Return to article