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Figure 6


Fig. 6. Surface reconstruction of a cell moving under agarose. Ax2 cells expressing cAR1-GFP were induced to chemotax under 0.6% agarose containing cAMP. Stacks of confocal sections were taken at the indicated times and reconstructed using the Volocity package. (A) A single confocal section through a cell at different times; (B) a maximum density projection of the z-section stack; (C) the 3D reconstruction viewed from 30° to the substratum; (D) the 3D reconstruction viewed from -30° to the substratum. The relative surface area and volume (A/V) of the cell are given in C. See Movie 11 in supplementary material.





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